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Results for entries tagged with "Scaffold & Biomaterial Assessment"
Large field-of-view Analysis of Fracture Callus HealingIn order to assess the extent of healing following non-union fracture in response to various therapies (PTH, bisphosphonates, etc), the callus sites of histologically stained (i.e. Safranin-O/FAST Green) and sectioned long bones can be digitized using a high resolution, large field-of-view microscope and subsequently evaluated for thickness, area, and tissue composition (fibrous, bone, cartilagenous tissue content) in a quati.
Fracture Union AnalysisNon-union fractures can heal in an amorphous fashion and are thus difficult to assess qualitatively with regard to bridging success. Leveraging the 3D output of micro-computed tomography (CT) and subsequent image analysis, it is however possible to quantitatively represent bridging or union in a more consistent and logical manner that accounts for the non-uniformity in healing.
Bright Field Immuno-StainingNon-fluorescent, immuno-staining of histological tissue for specific antigens generally involves enzymatic subtrates that oxidize compounds such as diaminobenzidine (DAB) to precipitate a brown chromogenic product. Unfortunately, "brown" color is composed of a mixture of hues not easily segmented or characterized from background.
Osseointegration of Bone Graft Analysis in CTAssessment of bone growth into scaffolds or grafts is generally performed qualitatively and thus difficult to consistently replicate across timepoints for a given patient or across multiple patients. This is especially true when CT imaging protocols (resolution, tube potential/current, etc) aren't kept consistent.
Osseointegration of Bone Scaffolds in a Canine Defect ModelThe canine femoral multi-defect model enables evaluation of bone in-growth within 4 separate defects placed > 1.5 cm apart in the proximal femur. For this model, animals are euthanized 4 weeks following implantation and bone scaffolds inserted into the surgically created cylindrical defects (1.0 cm diameter, 1.5 cm deep) are explanted and imaged using micro-computed tomography (CT).
Osseointegration in Bone SubstitutesTo determine efficacy of candidate bone substitutes for non-weight bearing applications such as facial reconstruction, rate and extent of osseointegration or mineralization are key assessment parameters. Unfortunately traditional means of evaluation require multiple animals to be sacrificed at each time point followed by exhaustive histological sectioning, staining, imaging and quantitative analysis for bone in-growth for the duration of the study.
Osseointegration of Titanium Bone ScrewsTo assess the effects of implant surface contaminants on osseointegration, titanium bone screws with variable degrees of cleaning were implanted into the mid-diaphysis of a mouse femur for 5 weeks, explanted and imaged using micro-CT. Subsequent customized image analysis using spectral and morphological filters enabled generation of 3D screw masks.
Micro-Computed Tomography (CT) Evaluation of Cartilage Profile using the Contrast Agent ConrayEvaluation of articular cartilage defects or delamination in small animal models for assessment of repair or response to pharmaceuticals requires both high spatial and density resolution. Unfortunately while small animal MRI systems will allow visualization cartilage, resolution is poor. While micro-computed tomography will provide the necessary resolution (as high as 1 um), soft tissue interfaces like cartilage do not attenuate well and thus are not generally visible or extremely noisy at best.
Trabecular Analysis on Human Bone CoresTrabecular bone quality assessment is generally not performed in patient CT scans due to inadequate resolution, however, bone cores may be extracted from patients in non-weight bearing sites (i.e. illiac crest) or from discards obtained during implant insertion and scanned using micro-computed tomography (micro-CT) for analysis of structural indices.
Automated Histological Analysis of Cartilage DefectsSports-related injuries can produce large focal defects in articular joint cartilage that often lead to delamination of surrounding healthy cartilage and eventual degeneration of the joint. To study this process in an animal model, a pendulum was swung onto the medial condyles of a rabbit femur. After a predefined length of time, the rabbit was sacrificed and the impact region was explanted, sectioned, and stained with Safranin-O.
Automated Histological Analysis of OsseointegrationThe structure and chemical composition of bone scaffolds or implants will impact their ability to promote bone in-growth and consequently dictate stability of the implant. Small animal models are often utilized for evaluation of bone in-growth in candidate scaffolds. Scaffolds are generally implanted into proximal sites of long bones for a fixed period of time.
Custom Software Development for Total Shoulder Replacement SurgeryTraditional tools for shoulder arthroplasty planning include evaluation of 2D x-ray micrographs, 3D CT volumes and/or MRI volumes in the operating room for estimation of glenoid vault dimensions and available mass for implant fixation
Scaffold/Implant Quality AssessmentEvaluation of devices pre and post implantation can be extremely valuable for assessing wear and degradation or just as a means of quality assurance. This is particularly important for biological scaffolds that have variable geometries post-fabrication. For such an assessment to be valuable, especially for samples evaluated pre-implantation, the imaging modality utilized has to be non-destructive and conducted in a sterile environment.
Osseointegration of Resorbable ACL ScrewsBioresorbable interference screws are theorized to fill in with bone tissue over time as they dissolve, but still provide the same initial anchoring support for the ACL as traditional metal screws. Potentially, such an implant could delay/eliminate revisions related to loosening that is observed with metallic implants.
Cartilage Thickness in CT ArthrogramsCT arthrograms involve injection of a contrast agent into joint spaces prior to scanning to study abnormalities or tears in articulating surfaces (cartilage), tendons, ligaments, or the joint capsule as a result of injury, osteoarthritis, or other pathology. Additionally, joint contrast will clearly delineate boundaries of soft tissue that generally is weakly attenuating and noisy.
- Bright Field Microscope (19)
- CT Scanner (18)
- Clinical MRI Scanner (2)
- Clinical PET Scanner (1)
- Clinical Ultrasound (1)
- Clinical X-Ray (3)
- Confocal Microscope (16)
- Electron Microscope (1)
- Fluorescence Microscope (15)
- High Resolution and High Throughput Microscope (19)
- Large Field of View Microscope (18)
- Micro-CT Scanner (17)
- Scanning Electron Microscope (3)
- Small Animal PET Scanner (1)
- 2D Image Processing - Analysis and Visualization (22)
- 3D Volumetric Imaging (1)
- 3D Volumetric Imaging & Analysis (18)
- Cell-Based Assays (13)
- Custom Software Development (39)
- Imaging and Image Analysis Study Consultation (38)
- Implant Assessment (12)
- Live Cell Imaging & Analysis (9)
- Microscopy Imaging & Analysis (20)
- Object Classification & Segmentation (40)
- Scaffold & Biomaterial Assessment (15)
- Time & Motion Analysis (25)
- Tissue-Based Assays (15)
- Cardiovascular (16)
- Dentistry (13)
- Dermatology - Plastic Surgery (15)
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- Orthopedic (30)
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- Radiology (23)
- Stem Cell / Gene Therapy (36)
- Surgery (30)
- Urology (5)