Search Results by Classification Category

Results for entries tagged with "Ophthalmology"

Bright Field Immuno-Staining

Non-fluorescent, immuno-staining of histological tissue for specific antigens generally involves enzymatic subtrates that oxidize compounds such as diaminobenzidine (DAB) to precipitate a brown chromogenic product. Unfortunately, "brown" color is composed of a mixture of hues not easily segmented or characterized from background.

Multi-modality Volumetric Registration

Spatial registration of longitudinal volumes and subsequent analysis can enable morphometric tracking of implants to evaluate degradation/biocompatibility or assess response of various pathologies/phenotypes to therapeutics or genetic manipulation.

Cell Proliferation/Lineage Analysis

Cellular division and lineage tracing analysis is an extremely complex problem with respect to image analysis but essential for cell fate assessment and phenotyping following genetic manipulation or drug treatment.

Automated Fundus Image Analysis

Fundus photography of the eye enables visualization of the retina, optic disc, fovea, and macula. Additionally it is the only place on the body where microcirculation can be observed non-invasively. A number of pathologies can be evaluated in fundus images including diabetic retinopathy, AMD (dry and wet), various tumors, glaucoma, vascular occlusion, etc.

Retinal Vascularization in Small Animal Models

A number of ophthalmic pathologies can be studied in small animals (rats and mice) to assess progression of disease following drug or gene therapy. This approach calls for the perfusion of retinal vasculature with a fluorescent dye, animal sacrifice, and "flat-mounting" of the explanted retina onto a slide (four radial incisions).

Tube Formation Analysis

Endothelial cells (EC), when provided with a 3D extracellular matrix substrate and appropriately supplemented growth media, will self-organize into a network of capillary-like "tubes." This in-vitro, "tube formation" assay, is commonly used to assess ability of compounds to stimulate or inhibit angiogenesis.

Cell/Vessel Proximity Assay

Since tumor survival is strongly dependent on vascularization, there are number of anticancer drugs that specifically target vessel growth. A subset of these drugs bind to growth factors (i.e. VEGF) and prevent them from binding to receptors that initiate angiogenesis cascades.