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Results for entries tagged with "Obstetrics and Gynaecology"
Cellular Particle Exclusion AssayCellular matrix production is crucial in the formation, maintenance, and repair of organs and tissues. While there are many dyes and antibodies available to study matrix production in-vitro, such reporters can generally only be applied as an endpoint tool as they require cell fixation and do not support live cell study.
Colony AnalysisUndifferentiated embryonic stem cells and induced pluripotent stem cells will form aggregates or "colonies" in vitro prior to differentiation. Evaluation of colony shape, area, cell density, rate of growth/proliferation, migration, etc. can be important factors in selection of an appropriate cells for subsequent differentiation and expansion for transplantation or bioassay development.
Bright Field Immuno-StainingNon-fluorescent, immuno-staining of histological tissue for specific antigens generally involves enzymatic subtrates that oxidize compounds such as diaminobenzidine (DAB) to precipitate a brown chromogenic product. Unfortunately, "brown" color is composed of a mixture of hues not easily segmented or characterized from background.
Multi-modality Volumetric RegistrationSpatial registration of longitudinal volumes and subsequent analysis can enable morphometric tracking of implants to evaluate degradation/biocompatibility or assess response of various pathologies/phenotypes to therapeutics or genetic manipulation.
Confocal Time-Lapse Imaging of Actin RufflesMechanics of endothelial cell (EC) migration have been extensively studied to elucidate mechanisms/pathways involved wound healing and neovascularization. It is widely accepted that actin, a major cytoskeletal component, plays a crucial role EC translocation.
Tube Formation AnalysisEndothelial cells (EC), when provided with a 3D extracellular matrix substrate and appropriately supplemented growth media, will self-organize into a network of capillary-like "tubes." This in-vitro, "tube formation" assay, is commonly used to assess ability of compounds to stimulate or inhibit angiogenesis.
- Bright Field Microscope (19)
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