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Results for entries tagged with "Electron Microscope"

Cellular Particle Exclusion Assay

Cellular matrix production is crucial in the formation, maintenance, and repair of organs and tissues. While there are many dyes and antibodies available to study matrix production in-vitro, such reporters can generally only be applied as an endpoint tool as they require cell fixation and do not support live cell study.

Determining Wear and Sharpness of Razor Blades Using a Scanning Electron Microscope

Analysis of wear and sharpness of cutting tools requires nanometer-level resolution for accurate assessment. Unfortunately there are limited choices of imaging modalities that can achieve such resolution. Scanning electron microscopy (SEM) is a common choice for such an evaluation, especially when the test surface is conductive and does not require metallic (sputter) coating. This was the case for a study in which wear of razor blades was a required output metric.

Colony Analysis

Undifferentiated embryonic stem cells and induced pluripotent stem cells will form aggregates or "colonies" in vitro prior to differentiation. Evaluation of colony shape, area, cell density, rate of growth/proliferation, migration, etc. can be important factors in selection of an appropriate cells for subsequent differentiation and expansion for transplantation or bioassay development.

TEM Collagen Fibril Morphometry

Transmission electron microscopy (TEM) is a valuable imaging tool for nanometer scale assessment of extracellular matrix organization and localization/morphology of subcellular structures. As an example, collagen fibrils in a genetically modified animal model were imaged using TEM for evaluation of collagen morphology and spacing/packing.